CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology is a relatively facile method for editing genes. Editing allows researchers to elucidate the function of genes, leading to new products and
procedures with benefits to human health and well being. Gene-edited cells are a useful tool for understanding disease processes and how we might intervene.
The genes of these cells may be overexpressed, knocked-out, or otherwise
edited in various ways to mimic diseased cells that will respond to experimental treatment as would the tissues being modeled.
The Genome Editing Core Facility offers a suite of services to support researchers who use these kinds of cells:
Clients who wish to avail the services of the GEF must accomplish the following forms:
The accomplished forms must be sent to firstname.lastname@example.org with the subject GEF Service: [Name of Lab/Institute]. Please wait for a confirmation receipt and the billing statement for the required service. GEF will only commence the service once the official receipt from the UPD Cash Office has been issued.
For clients who wish to avail of transduction services, additional documents and forms are required for each cell line sample submission:
At least 1-mL of cryogenically stored cell solution must be submitted prior to generation of lentiviral particles. Samples must adhere to the triple-packaging system based on the WHO biosafety guidelines. Samples must also be transported in appropriate storage conditions (ie with dry ice).
For sub-cloning of target genes into lentiviral overexpression vectors, entry plasmids must be dissolved in nuclease-free water at a concentration of 200ng/μL with volume not lower than 50μL. Please secure the microfuge tube with parafilm prior to transport.