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About

Since its establishment in 1997, the laboratory has been providing research training for undergraduate and graduate NIMBB students. These activities have resulted in several publications and paper presentations in local and international scientific journals and conferences, all co-authored by students.

The Molecular Microbiology Laboratory (MML) is the center of activities for molecular microbiology and microbial biotechnology research at the NIMBB UP Diliman.

Published Projects
Polymerase chain reaction-based detection of water-borne bacterial pathogens

The analysis of complete gene sequences from bacterial shrimp pathogens Vibrio harveyi and Vibrio campbelliiThe identification of genetic markers to identify alcohol-tolerant wine strains of the yeast Saccharomyces cerevisiae.

Current projects
The detection of enzyme genes from thermophilic bacteria isolated from a variety of sources. The isolation of bunker oil-utilizing bacteria from oil sludge and the subsequent cloning and expression of genes implicated in oil utilization.

Other completed studies
DNA-based detection of genetically modified corn and soybean seeds. Sequence analysis of mitochondrial 16s rRNA and cytochrome b genes in selected Philippine wildlife species.

The Molecular Microbiology Laboratory continues to conduct molecular microbial-based research activities and to mentor students to complete theses and dissertations on the elucidation of virulence factors in bacterial pathogens, the detection and isolation of genes for enzymes implicated in bioremediation and other industrial applications, and the analysis of gene sequences crucial to the classification and identification of bacteria.

People

Project Investigator
Cynthia T. Hedreyda, PhDCTH

Publications

Hedreyda, C. T., & Sarmago, M. G. (2014). Detection and sequence analysis of the alkM gene in Acinetobacter baumannii strain OS1 from oil sludge sample. Philippine Science Letters, 7(1), 1-6.

Hedreyda, C. T., Dominguez, J. J. A., & Rosal, K. (2012). Isolation of thermophilic bacteria (Bacillus and Ureibacillus) and amplification of genes for selected enzymes. Philippine Science Letters, 5(2), 209-215

Guiamal, Z. J. A., & Hedreyda, C. T. (2011). Microsatellite and RAPD Analysis of Six Local Wine Strains of saccharomyces cerevisiae. Philippine Science Letters, 4(2), 122-130.

Hedreyda, C. T., & Orata, F. D. (2011). Amplification and sequence analysis of the luxA gene in the non-luminescent type strain Vibrio campbellii NBRC 15631. Philippine Science Letters, 4(2), 114-121.

Orata, F. D., & Hedreyda, C. T. (2011). Isolation and sequence analysis of the full-length toxR gene of type strain Vibrio campbellii and use of the toxR gene sequence to evaluate variation and relatedness with other Vibrio species. Philippine Science Lettrs, 4(1), 70-80.

De Leon, M. I. A. Z. Ponce; Gonzalez, Juan Carlos T.; Hedreyda, C. T. (2008). Complete DNA sequence analysis of the mitochondrial 16s rRNA and cytochrome b genes of the Visayan warty pig (Sus cebifrons). ASIA LIFE SCIENCES, 17(2), 295-308.

Hedreyda, C. T., & Rañoa, D. R. E. (2007). Sequence analysis of the Vibrio harveyi ornithine decarboxylase (odc) gene and detection of a gene homologue in Vibrio campbellii. The Journal of general and applied microbiology, 53(6), 353-6.

Azanza, M. P. V., Azanza, R. V., Vargas, V. M. D., & Hedreyda, C. T. (2006). Bacterial endosymbionts of Pyrodinium bahamense var. compressum. Microbial ecology, 52(4), 756-64. doi:10.1007/s00248-006-9128-7

Castroverde, C. D. M., San Luis, B. B., Monsalud, R. G., & Hedreyda, C. T. (2006). Differential detection of vibrios pathogenic to shrimp by multiplex PCR. The Journal of general and applied microbiology, 52(5), 273-80.

Franco, P. F., & Hedreyda, C. T. (2006). Amplification and sequence analysis of the full length toxR gene in Vibrio harveyi. The Journal of general and applied microbiology, 52(5), 281-7.

Rañoa, D. R. E., & Hedreyda, C. T. (2006). Sequence analysis of partial toxR gene from Philippine Vibrio isolates and design of toxR-targeted primers for detection. The Journal of general and applied microbiology, 51(6), 343-51.

San Luis, B. B., & Hedreyda, C. T. (2006). Analysis of a gene (vch) encoding hemolysin isolated and sequenced from Vibrio campbellii. The Journal of general and applied microbiology, 52(6), 303-13.

Conejero, M. J. U., & Hedreyda, C. T. (2004). PCR detection of hemolysin (vhh) gene in Vibrio harveyi. The Journal of general and applied microbiology, 50(3), 137-42.

Conejero, M. J. U., & Hedreyda, C. T. (2003). Isolation of partial toxR gene of Vibrio harveyi and design of toxR-targeted PCR primers for species detection. Journal of Applied Microbiology, 95(3), 602-611. doi:10.1046/j.1365-2672.2003.02020.x

Monsalud, R. G., Magbanua, F. O., Tapay, L. M., Hedreyda, C. T., Olympia, M. S. D., Migo, V. P., Kurahashi, M., et al. (2003). Identification of pathogenic and non-pathogenic Vibrio strains from shrimp and shrimp farms in the Philippines. The Journal of General and Applied Microbiology, 49(5), 309-314.